Glöggler S, Wagner S, Bouchard LS. Hyperpolarization of amino acid derivatives in water for biological applications. Chemical Science. 2015.
Glöggler S, Grunfeld AM, Ertas YN, McCormick J, Wagner S, Schleker PPM, et al. A Nanoparticle Catalyst for Heterogeneous Phase Para-Hydrogen-Induced Polarization in Water. Angewandte Chemie. 2015;127(8):2482-6.
Wagner S. Conversion rate of para-hydrogen to ortho-hydrogen by oxygen: implications for PHIP gas storage and utilization. Magn Reson Mater Phy. 2014;27(3):195-9.
Agraz J, Grunfeld A, Li D, Cunningham K, Willey C, Pozos R, et al. LabVIEW-based control software for para-hydrogen induced polarization instrumentation. Review of Scientific Instruments. 2014;85(4):044705.
Agraz J, Grunfeld A, Cunningham K, Li D, Wagner S. Improved PHIP polarization using a precision, low noise, voltage controlled current source. Journal of Magnetic Resonance. 2013;235(0):77-84.
Targeted Multifunctional Nanoparticles
Nano medicine is a rapidly growing field of interest offering new opportunities for drug delivery and disease imaging. We are developing a delivery platform utilizing selective targeting of cell surface receptors via binding with the protein surface layer. By using a unique method of self-assembly we can rapidly synthesis nanoparticles with precise sizes.
The primary focus of this project is to utilize the metal core to enhance radiation effectiveness by accumulation of the metal nanoparticles near the nuclei of cancer cells. A high percentage of breast-brain metastatic cancers have highly elevated levels of HER2 on the membrane surface. By targeting a receptor we can selectively delivery the nanoparticles to cancer cells elevating effectiveness of whole brain radiation therapy.
Our secondary objective is to utilize the metal core (Ag; silver) for magnetic resonance imaging. We are investigating if solid state dynamic nuclear polarization (DNP) can be utilized to imaging the particle in vivo as a method of cancer detection. The unique design of our particle provides the required unpaired electron for the DNP process to hyperpolarize the silver, and with an approximate 50:50 split of two ½ nuclei (107Ag and 109Ag) we can image utilizing two different hyperpolarization pools providing the opportunity to image twice by changing the resonance frequency.
Parahydrogen Induced Hyperpolarization (PHIP)
The potential to investigate disease metabolism in vivo is a valuable tool. Past NMR research has focused on delivering high concentrations of 13C enriched metabolites and long acquisitions time to assess metabolic use. With new technology this information can be obtained in minutes with low concentration metabolic probes.
Hyperpolarization enhances the NMR/MRI signal 10,000-30,000 enabling the detection of real time metabolism in vivo using metabolic probes. We have developed a sterile polarization instrument with precise sample control using custom hardware/ software. Our system allows for rapid chemical mixing, polarization and ejection of the sample (~3 seconds). Additionally, we are collaborating to develop and test new heterogeneous catalysts to replace the standard Wilkinson catalyst providing the opportunity to remove the catalyst from the hyperpolarized compound.